7WR3
Crystal structure of MBP-fused OspC3 in complex with calmodulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2021-05-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.846, 201.866, 101.515 |
Unit cell angles | 90.00, 102.11, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.990 | 1.920 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.088 | 0.865 |
Rmeas | 0.096 | 0.939 |
Number of reflections | 187465 | 13706 |
<I/σ(I)> | 12.49 | 2.29 |
Completeness [%] | 99.2 | 98.2 |
Redundancy | 6.86 | |
CC(1/2) | 0.998 | 0.736 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG3350, 0.1 M Tris pH 8.2-8.4, 0.2 M Lithium Sulfate, 0.7% 1-Butanol |