7WNS
Crystal structure of ENF peptide binding protein from Silkworm
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 54.477, 121.692, 253.769 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.543 - 1.799 |
Rwork | 0.173 |
R-free | 0.18530 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.997 |
Data reduction software | HKL-3000 |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.799 | 1.800 |
Rmerge | 0.082 | 0.928 |
Number of reflections | 78393 | 3823 |
<I/σ(I)> | 36.67 | 3 |
Completeness [%] | 99.5 | 99 |
Redundancy | 13.2 | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | O.5M magnesium formate, 0.1M Bis-Tris pH 6.5 |