7VX2
Crystal Structure of the Y53F/N55A/I80F/L114V/I116V mutant of LEH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-10-30 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1.0331 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.154, 84.039, 93.371 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.801 - 2.485 |
R-factor | 0.2336 |
Rwork | 0.231 |
R-free | 0.27690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nww |
RMSD bond length | 0.003 |
RMSD bond angle | 0.550 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.574 |
High resolution limit [Å] | 2.485 | 2.485 |
Number of reflections | 20046 | 1838 |
<I/σ(I)> | 9.59 | 3.35 |
Completeness [%] | 94.6 | 89.47 |
Redundancy | 6.9 | 6.7 |
CC(1/2) | 0.994 | 0.926 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | Ammonium acetate, Polyethylene glycol 3350 |