7VWO
TA complex from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.847, 74.501, 74.882 |
| Unit cell angles | 73.16, 70.24, 70.03 |
Refinement procedure
| Resolution | 44.550 - 2.000 |
| R-factor | 0.2036 |
| Rwork | 0.201 |
| R-free | 0.24685 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.936 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.550 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 | |
| Rmeas | 0.064 | |
| Rpim | 0.042 | |
| Number of reflections | 59128 | 8216 |
| <I/σ(I)> | 5.1 | |
| Completeness [%] | 95.7 | |
| Redundancy | 2 | |
| CC(1/2) | 0.996 | 0.323 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Potassium chloride, 0.1 M Magnesium acetate tetrahydrate 0.05 M Sodium cacodylate trihydrate pH 6.5 10% w/v Polyethylene glycol 8,000 |
