7VPW
Crystal structure of Transportin-1 in complex with BAP1 PY-NLS (residues 706-724)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-03-10 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 68.896, 129.688, 170.214 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.860 - 3.760 |
R-factor | 0.2324 |
Rwork | 0.229 |
R-free | 0.25840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z5o |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 103.200 | 3.880 |
High resolution limit [Å] | 3.760 | 3.760 |
Rmerge | 0.210 | |
Rpim | 0.090 | |
Number of reflections | 16059 | 795 |
<I/σ(I)> | 7.3 | |
Completeness [%] | 99.6 | |
Redundancy | 6.88 | |
CC(1/2) | 0.905 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | 0.49 M Sodium phosphate monobasic monohydrate, 0.91 M Sodium phosphate dibasic, pH 6.9 |