7VNL
Sandercyanin mutant-F55A-Biliverdin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-07-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9897 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 158.388, 158.388, 81.073 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.360 - 1.930 |
R-factor | 0.1949 |
Rwork | 0.193 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ez2 |
RMSD bond length | 0.008 |
Data reduction software | DIALS |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHENIX (1.15.2-3472) |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.120 | 2.030 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmeas | 0.229 | |
Rpim | 0.026 | 0.581 |
Number of reflections | 45351 | 6479 |
<I/σ(I)> | 20.8 | |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 74.4 | 74.8 |
CC(1/2) | 1.000 | 0.586 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1M sodium malonate pH 7, 12% PEG w/v 3350 |