7VMJ
Crystal structure of tubulin with 17a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-23 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.976, 157.934, 182.334 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 87.430 - 2.900 |
R-factor | 0.2002 |
Rwork | 0.199 |
R-free | 0.24340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i55 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 91.170 | 91.170 | 2.970 |
High resolution limit [Å] | 2.900 | 13.600 | 2.900 |
Rmerge | 0.245 | 0.089 | 2.049 |
Rmeas | 0.266 | 0.099 | 2.219 |
Rpim | 0.102 | 0.041 | 0.846 |
Total number of observations | 452530 | 3797 | 30619 |
Number of reflections | 67923 | 760 | 4490 |
<I/σ(I)> | 5.8 | 7.8 | 1.6 |
Completeness [%] | 100.0 | 98.6 | 100 |
Redundancy | 6.7 | 5 | 6.8 |
CC(1/2) | 0.984 | 0.988 | 0.503 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 6% polyethylene glycol 4000, 8% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7 |