7VD7
Toxin - Antitoxin complex from Salmonella enterica serovar Typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97959 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.366, 31.605, 47.985 |
| Unit cell angles | 90.00, 90.63, 90.00 |
Refinement procedure
| Resolution | 24.000 - 1.430 |
| R-factor | 0.19664 |
| Rwork | 0.196 |
| R-free | 0.21615 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | no PDB entry |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.730 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.19.2-4158) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.460 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Number of reflections | 20736 | 1569 |
| <I/σ(I)> | 49.02 | |
| Completeness [%] | 93.1 | |
| Redundancy | 6.4 | 5.7 |
| CC(1/2) | 0.999 | 0.975 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (w/v) PEG 6000 100 mM HEPES/ Sodium hydroxide pH 7.0 200 mM Sodium chloride |
