7VBR
Fe(II)/(alpha)ketoglutarate-dependent dioxygenase TlxI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-20 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 |
Unit cell lengths | 64.403, 64.450, 76.988 |
Unit cell angles | 81.75, 73.70, 74.83 |
Refinement procedure
Resolution | 49.320 - 2.100 |
R-factor | 0.2101 |
Rwork | 0.209 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zm2 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.911 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.320 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.081 | 0.711 |
Number of reflections | 65297 | 4485 |
<I/σ(I)> | 9.1 | 1.5 |
Completeness [%] | 97.7 | |
Redundancy | 3.7 | |
CC(1/2) | 0.996 | 0.601 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M Bis-Tris (PH 6.5), 2% (v/v) Tacsimate (PH 6.0), 20% (w/v) PEG 3350 |