7VA8

Crystal structure of MiCGT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-15
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	92.390, 104.680, 109.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.742 - 2.850
R-factor	0.263633226401
Rwork	0.262
R-free	0.28641
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vce
RMSD bond length	0.004
RMSD bond angle	0.885
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.750	2.920
High resolution limit [Å]	2.850	2.850
Number of reflections	25435	25435
<I/σ(I)>	6.6
Completeness [%]	99.7
Redundancy	6.2
CC(1/2)	0.986	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% (v/v) PEG3350, 0.1 M Bis-Tris pH 6.5, 200 mM calcium acetate and 4% (v/v) formamide were