7V9H
The BEN3 domain of protein Bend3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97885 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 54.011, 54.011, 70.772 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.775 - 2.692 |
| R-factor | 0.1815 |
| Rwork | 0.180 |
| R-free | 0.21890 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.649 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.159 | 0.159 |
| Number of reflections | 3542 | 335 |
| <I/σ(I)> | 87.9 | |
| Completeness [%] | 99.4 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 1.0 M DL-Malic acid, pH 7.0 and 0.1 M BIS-TRIS propane, pH 7.0 |
