7V6P
Crystal structure of human sNASP TPR domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 96.833, 96.833, 209.333 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.900 |
R-factor | 0.1883 |
Rwork | 0.187 |
R-free | 0.22220 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 0.925 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((1.19.2_4158)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.061 | 0.876 |
Number of reflections | 13286 | 2280 |
<I/σ(I)> | 31.1 | 2.6 |
Completeness [%] | 98.5 | |
Redundancy | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M Sodium cacodylate, pH 6.5 32% (v/v) PEG 300 0.2 M calcium acetate |