7V51
BVMO_negative mutant D432V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2018-10-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.340, 53.405, 102.191 |
| Unit cell angles | 90.00, 97.05, 90.00 |
Refinement procedure
| Resolution | 39.090 - 2.420 |
| R-factor | 0.1925 |
| Rwork | 0.190 |
| R-free | 0.24740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6a37 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.023 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.066 | 0.066 |
| Number of reflections | 21933 | 431919 |
| <I/σ(I)> | 15.628 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | HEPES, PEG4000, CaCl2 |
