7V1Q
Leifsonia Alcohol Dehydrogenases LnADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.671, 238.522, 68.921 |
| Unit cell angles | 90.00, 113.35, 90.00 |
Refinement procedure
| Resolution | 49.560 - 1.580 |
| R-factor | 0.1657 |
| Rwork | 0.164 |
| R-free | 0.19530 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.364 |
| Data scaling software | HKL-2000 |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.149 | 0.559 |
| Number of reflections | 219460 | 11079 |
| <I/σ(I)> | 15.7 | 3.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M Sodium acetate/Acetic acid, pH 7.0, 0.2M Zinc acetate, 20% PEG 1000 |
