7UDZ
Designed pentameric proton channel LQLL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.03318 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 86.772, 46.658, 66.320 |
Unit cell angles | 90.00, 108.95, 90.00 |
Refinement procedure
Resolution | 36.870 - 2.480 |
R-factor | 0.2488 |
Rwork | 0.248 |
R-free | 0.26930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mct |
RMSD bond length | 0.002 |
RMSD bond angle | 0.414 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.030 | 2.610 |
High resolution limit [Å] | 2.470 | 2.470 |
Rmerge | 0.708 | |
Number of reflections | 8896 | 1248 |
<I/σ(I)> | 8.1 | |
Completeness [%] | 98.1 | |
Redundancy | 3.2 | |
CC(1/2) | 0.991 | 0.800 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293.15 | 0.1 M calcium chloride, 0.1 M MES, 29% v/v PEG400 |