7UDY

Designed pentameric channel QLLL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03318
Spacegroup name	C 2 2 21
Unit cell lengths	56.385, 84.837, 149.557
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.770 - 2.400
R-factor	0.2307
Rwork	0.230
R-free	0.25070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mct
RMSD bond length	0.001
RMSD bond angle	0.324
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.780	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.155	1.249
Number of reflections	14395	2034
<I/σ(I)>	8.1
Completeness [%]	99.5
Redundancy	8.5
CC(1/2)	0.998	0.831

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293.15	0.22 M sodium citrate, 0.1 M Tris, pH 8.0, 35% v/v PEG400