7UC7
Stat5a Core in complex with Compound 17
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-03-24 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9787 |
Spacegroup name | H 3 2 |
Unit cell lengths | 238.895, 238.895, 113.304 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.880 - 3.102 |
R-factor | 0.2248 |
Rwork | 0.222 |
R-free | 0.28140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | previously solved structure in lab |
RMSD bond length | 0.009 |
RMSD bond angle | 1.030 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4 (3-FEB-2022)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
Rmerge | 0.076 | 0.035 | 1.515 |
Rmeas | 0.079 | 0.037 | 1.586 |
Rpim | 0.024 | 0.012 | 0.466 |
Total number of observations | 256562 | ||
Number of reflections | 22460 | 1168 | 1101 |
<I/σ(I)> | 7 | ||
Completeness [%] | 99.9 | 98.8 | 100 |
Redundancy | 11.4 | 10.3 | 11.6 |
CC(1/2) | 0.997 | 0.914 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.1M Ammonium Tartrate dibasic pH 7.0 |