7UBU

Crystal structure of ZMET2 in complex with hemimethylated CAG DNA and a histone H3Kc9me2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2019-11-18
Detector	AGILENT ATLAS CCD
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	64.574, 121.392, 160.446
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	96.810 - 2.390
R-factor	0.2132
Rwork	0.211
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ft2
Data reduction software	HKL-3000
Data scaling software	HKL-3000 (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	160.450	160.450	2.470
High resolution limit [Å]	2.390	9.560	2.390
Rmerge	0.129	0.036	1.873
Rmeas	0.143	0.040	2.058
Rpim	0.062	0.018	0.840
Total number of observations	286070	4549	27542
Number of reflections	50383	905	4604
<I/σ(I)>	10.1	32.7	0.8
Completeness [%]	99.3	98.9	99.9
Redundancy	5.7	5	6
CC(1/2)	0.992	0.997	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.1 M sodium citrate pH 5.5, 15% w/v polyethylene glycol 6,000