7U1M
Crystal structure of NTMT1 in complex with compound YD206
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 65 |
| Unit cell lengths | 89.609, 89.609, 141.502 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.800 - 3.170 |
| R-factor | 0.2538 |
| Rwork | 0.251 |
| R-free | 0.28280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pva |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18rc1_3777) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rpim | 0.072 | 0.927 |
| Number of reflections | 11983 | 1196 |
| <I/σ(I)> | 29.8 | 1 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 6.9 | 8.2 |
| CC(1/2) | 1.000 | 0.426 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M CHES:NaOH, pH 9.5 10% (w/v) PEG 3000 |
