Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

7TWA

Crystal structure of apo BesC from Streptomyces cattleya

Experimental procedure
Experimental methodMAD
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 8.3.1
Synchrotron siteALS
Beamline8.3.1
Temperature [K]194
Detector technologyPIXEL
Collection date2016-12-14
DetectorDECTRIS PILATUS3 S 6M
Wavelength(s)0.953680
Spacegroup nameP 1 21 1
Unit cell lengths60.917, 68.977, 126.748
Unit cell angles90.00, 89.99, 90.00
Refinement procedure
Resolution126.748 - 1.700
R-factor0.2355
Rwork0.199
R-free0.24710
Structure solution methodMAD
RMSD bond length0.004
RMSD bond angle0.540
Data reduction softwareXDS
Data scaling softwareAimless (0.7.4)
Phasing softwarePHENIX (1.13)
Refinement softwarePHENIX (1.13_2998)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]126.750126.7501.730
High resolution limit [Å]1.7009.3101.700
Rmerge0.0800.0610.635
Rmeas0.0850.0650.688
Rpim0.0290.0220.258
Total number of observations941610615336345
Number of reflections1153017555378
<I/σ(I)>14.232.92.6
Completeness [%]99.799.994.6
Redundancy8.28.16.8
CC(1/2)0.9990.9990.917
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.5293SeMet crystals were prepared by micro-seeding equal volumes of protein solution (10 mg/mL BesC in 50 mM HEPES pH 7.5 and 1 mM TCEP) and reservoir solution (calcium acetate (0.6 mM), PEG 3350 (25% (v/v)) and 1,3 butane-diol (4%))

221051

PDB entries from 2024-06-12

PDB statisticsPDBj update infoContact PDBjnumon