7TVY

Short form D7 protein from Culex quinquefasciatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-28
Detector	SBC-3
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	33.231, 122.809, 74.433
Unit cell angles	90.00, 90.43, 90.00

Refinement procedure

Resolution	33.230 - 1.980
R-factor	0.1944
Rwork	0.192
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold2 model
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.010
High resolution limit [Å]	1.980	5.370	1.980
Rmerge	0.064	0.029	0.421
Total number of observations	202477
Number of reflections	41251	2112	1822
<I/σ(I)>	13
Completeness [%]	99.4	99.2	89.2
Redundancy	4.9	4.8	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	25% PEG 5000 MME, 0.2 M lithium sulfate, 0.1 M Tris HCl, pH 8.5