7TVW
Crystal structure of Arabidopsis thaliana DLK2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.560, 84.590, 76.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.295 - 1.480 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.18840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ih1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.104 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.295 | 1.533 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.071 | 0.730 |
| Number of reflections | 43371 | 4281 |
| <I/σ(I)> | 17.42 | |
| Completeness [%] | 99.7 | 99.35 |
| Redundancy | 7.5 | 6.1 |
| CC(1/2) | 0.999 | 0.881 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris, 1.9 M ammonium sulfate |
