7TBB

Crystal structure of Plasmepsin X from Plasmodium falciparum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9536
Spacegroup name	P 21 21 21
Unit cell lengths	62.308, 63.152, 79.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.590 - 1.850
R-factor	0.2116
Rwork	0.208
R-free	0.25990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ux4
RMSD bond length	0.007
RMSD bond angle	0.946
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	Auto-Rickshaw
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.590	1.920
High resolution limit [Å]	1.850	1.850
Rpim	0.029	0.041
Number of reflections	27357	2688
<I/σ(I)>	15.71	1.43
Completeness [%]	99.9	99.9
Redundancy	13.3	13.4
CC(1/2)	1.000	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	25% (w/v) PEG1500, 10% (w/v) SPG pH 4.0