7SKZ
Crystal Structure of VN01H1 Fab in complex with SARS-CoV-2 S fusion peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2021-04-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9774 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 152.923, 60.713, 61.110 |
Unit cell angles | 90.00, 93.62, 90.00 |
Refinement procedure
Resolution | 46.240 - 1.860 |
R-factor | 0.2008 |
Rwork | 0.199 |
R-free | 0.23360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7r6w |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.240 | 1.900 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.077 | 1.600 |
Number of reflections | 46943 | 9477 |
<I/σ(I)> | 6.5 | |
Completeness [%] | 99.7 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 295 | 0.2M potassium acetate, 20% (w/v) PEG 3350, 0.2 M potassium chloride, 0.05 M HEPES-NaOH pH 7.5, 35% (v/v) pentaerythritol propoxylate 5/4/PO/OH |