7SGX
Crystal Structure of Turtle Cadherin-23 EC1-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 94.984, 94.984, 358.356 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.056 - 3.130 |
| Rwork | 0.253 |
| R-free | 0.31030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2whv |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.313 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.130 | 3.130 |
| Rmeas | 0.297 | 2.187 |
| Number of reflections | 30114 | 1456 |
| <I/σ(I)> | 12.559 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 277 | 0.1 M Tris pH 7.7 1.9 M CaCl2 |
