7SEG
Crystal structure of the complex of CD16A bound by an anti-CD16A Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2019-04-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.307, 220.560, 70.825 |
Unit cell angles | 90.00, 108.55, 90.00 |
Refinement procedure
Resolution | 33.885 - 2.156 |
R-factor | 0.1938 |
Rwork | 0.189 |
R-free | 0.23390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 14EJ |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 35.000 | 2.190 |
High resolution limit [Å] | 2.150 | 5.830 | 2.150 |
Rmerge | 0.098 | 0.068 | 0.552 |
Rmeas | 0.119 | 0.081 | 0.734 |
Rpim | 0.065 | 0.044 | 0.479 |
Total number of observations | 184828 | ||
Number of reflections | 64779 | 3475 | 2209 |
<I/σ(I)> | 6.1 | ||
Completeness [%] | 92.8 | 98.2 | 64.1 |
Redundancy | 2.9 | 3.4 | 1.7 |
CC(1/2) | 0.991 | 0.435 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 0.1M Bis-Tris pH5.5, 0.2M MgCl2, 0.01M Betaine HCl, 25% PEG3350 |