7S4V
Cas9 bound to 12-14MM DNA, 60 min time-point, kinked conformation
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.28 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
