7RM3
Antibody 2E10.E9 in complex with P. vivax CSP peptide ANGAGNQPGANGAGNQPGANGAGGQAA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-08-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.033167 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.404, 144.436, 60.471 |
Unit cell angles | 90.00, 102.83, 90.00 |
Refinement procedure
Resolution | 29.480 - 2.680 |
R-factor | 0.2081 |
Rwork | 0.206 |
R-free | 0.23940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uyw |
RMSD bond length | 0.009 |
RMSD bond angle | 1.241 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.480 | 2.780 |
High resolution limit [Å] | 2.680 | 2.680 |
Number of reflections | 26439 | 2627 |
<I/σ(I)> | 9.2 | 1.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7 | 7.1 |
CC(1/2) | 0.993 | 0.578 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M sodium cacodylate pH 6.5, 0.2 M sodium acetate and 30% (w/v) PEG 8000 |