7RCR
Crystal Structure of the refolded Hemagglutinin head domain of Influenza A virus A/Ohio/09/2015
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-03-04 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.670, 61.120, 89.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.060 - 1.600 |
R-factor | 0.1626 |
Rwork | 0.160 |
R-free | 0.19970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6uynA as per Morda |
RMSD bond length | 0.007 |
RMSD bond angle | 0.917 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MoRDa |
Refinement software | PHENIX (1.19 dev_4274) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.640 |
High resolution limit [Å] | 1.600 | 7.160 | 1.600 |
Rmerge | 0.074 | 0.030 | 0.597 |
Rmeas | 0.081 | 0.034 | 0.656 |
Number of reflections | 27045 | 337 | 1968 |
<I/σ(I)> | 16.87 | 39.16 | 3.4 |
Completeness [%] | 99.2 | 92.6 | 99.9 |
Redundancy | 5.99 | 5.264 | 6.005 |
CC(1/2) | 0.999 | 0.999 | 0.859 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 287 | RigakuReagents JCSG+ screen, condition a10: 200mM potassium formate, 20% (w/V) PEG 3350: InvbR.18715.a.TK11.PD38402 at 4.1mg/ml: tray: 314055a1: cryo: 20% EG: puck vjj0-9. |