7R22
Crystal structure of protein Mab3862 from Mycobacterium abscessus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-01 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 106.886, 106.886, 106.886 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.800 - 2.730 |
| R-factor | 0.32 |
| Rwork | 0.299 |
| R-free | 0.34730 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.045 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.880 | 2.780 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rmerge | 0.125 | |
| Number of reflections | 5863 | 296 |
| <I/σ(I)> | 26.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20 | |
| CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 298 | Sodium acetate pH 5.0 50mM Ammonium sulphate 2.0M |
