7QW5
Adenine-specific DNA methyltransferase M.BseCI complexed with AdoHcy and cognate unmethylated DNA duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-09 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.1 |
Spacegroup name | P 61 |
Unit cell lengths | 87.040, 87.040, 156.350 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.490 - 2.300 |
R-factor | 0.1986 |
Rwork | 0.197 |
R-free | 0.22380 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.731 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.910 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 38301 | 3358 |
<I/σ(I)> | 22.8 | 2 |
Completeness [%] | 99.5 | 98.5 |
Redundancy | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | PEG 400, Tris-HCl, KCl, magnesium chloride |