7QOA
Structure of CodB, a cytosine transporter in an outward-facing conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 108.246, 209.027, 102.452 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.160 - 2.400 |
R-factor | 0.2201 |
Rwork | 0.218 |
R-free | 0.26520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jln |
RMSD bond length | 0.007 |
RMSD bond angle | 0.904 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | MR-Rosetta |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.160 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.374 | |
Number of reflections | 45754 | 4436 |
<I/σ(I)> | 11.57 | |
Completeness [%] | 99.4 | |
Redundancy | 12.7 | |
CC(1/2) | 0.998 | 0.466 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6.5 | 293 | 0.1 M Sodium Cacodylate pH 6.5, 0.45 M NaCl, 39% PEG400 |