7QJ6
Structure of recombinant human gamma-Tubulin Ring Complex 10-spoked assembly intermediate (spokes 3-12, substoichiometric spokes 13-14)
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 7.8 |
| Number of Particle | 436887 |
| Software | Topaz,PHENIX,RELION,EPU,Gctf,NAMD,VMD |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
