7QBG
TC:CD320 in complex with nanobody TC-Nb4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979502 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.339, 116.418, 125.498 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.920 - 2.690 |
R-factor | 0.2299 |
Rwork | 0.227 |
R-free | 0.28540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zrp |
RMSD bond length | 0.011 |
RMSD bond angle | 2.025 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.920 | 2.790 |
High resolution limit [Å] | 2.690 | 2.690 |
Number of reflections | 84243 | 7177 |
<I/σ(I)> | 7.97 | |
Completeness [%] | 98.0 | |
Redundancy | 2 | |
CC(1/2) | 0.993 | 0.459 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 300 | 100 mM Bis-Tris pH 5.5, 25% w/v PEG 3350, supplemented with 10% (0.2% w/v Betaine anhydrous, 0.2% w/v L-Glutamic acid, 0.2% w/v L-Proline, 0.2% w/v Taurine, 0.2% w/v Trimethylamine N-oxide dihydrate, 0.02 M HEPES sodium pH 6.8 |