7Q9X
Crystal structure of Chromobacterium violaceum aminotransferase in complex with PLP-pyruvate adduct
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9700 |
Spacegroup name | P 1 |
Unit cell lengths | 60.330, 61.380, 116.480 |
Unit cell angles | 103.56, 89.38, 105.42 |
Refinement procedure
Resolution | 39.430 - 1.600 |
Rwork | 0.227 |
R-free | 0.26120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ba4 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.422 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.430 | 1.640 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 156999 | 3529 |
<I/σ(I)> | 14.9 | 2.5 |
Completeness [%] | 76.1 | 23 |
Redundancy | 4.7 | 4.3 |
CC(1/2) | 0.999 | 0.861 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 290 | 12.5% PEG 8000 and 50 mM HEPES pH 7.5 10 mM PLP, 5 mM pyruvate |