7Q7X
Crystal structure of the methyltransferase-ribozyme 1 (with 1-methyl-adenosine)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9198 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 71.037, 71.037, 82.748 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.940 - 2.800 |
R-factor | 0.2147 |
Rwork | 0.213 |
R-free | 0.25530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7oax |
RMSD bond length | 0.001 |
RMSD bond angle | 0.198 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.940 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.110 | |
Number of reflections | 5592 | 533 |
<I/σ(I)> | 18.74 | 1.13 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 24.8 | 25.5 |
CC(1/2) | 0.996 | 0.664 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 36-42% MPD, 50mM MES pH 6.4-6.7, 100mM NaCl, 100mM LiCl, 10mM MgCl2 |