7Q7M
Room temperature structure of the Rhodobacter Sphaeroides Photosynthetic Reaction Center F(M197)H mutant at 100 MPa helium gas pressure in a sapphire capillary
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 295 |
Detector technology | PIXEL |
Collection date | 2019-10-06 |
Detector | DECTRIS PILATUS3 X CdTe 2M |
Wavelength(s) | 0.477 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 102.210, 102.210, 237.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.950 - 2.550 |
R-factor | 0.1844 |
Rwork | 0.183 |
R-free | 0.22260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3v3y |
RMSD bond length | 0.003 |
RMSD bond angle | 0.578 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13-2998_9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.950 | 2.641 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.509 | 2.931 |
Rmeas | 0.525 | 3.027 |
Rpim | 0.124 | 0.739 |
Number of reflections | 41962 | 4104 |
<I/σ(I)> | 5.93 | 1 |
Completeness [%] | 99.8 | 99.95 |
Redundancy | 17.2 | 16.2 |
CC(1/2) | 0.979 | 0.375 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 289 | 20-25 mg/ml protein solution was mixed 1:1 with lipid sponge phase. The mixture was filled into a sealed plastic tip covered with an excess amount of 1 M trisodium citrate and incubated in the dark at 289 K for 1 week. |