7Q74
Structure of Pla1 apo, with a C-terminal deletion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-25 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 |
Unit cell lengths | 71.790, 72.480, 73.210 |
Unit cell angles | 99.96, 105.70, 118.83 |
Refinement procedure
Resolution | 57.351 - 2.599 |
R-factor | 0.2141 |
Rwork | 0.212 |
R-free | 0.25750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hhp |
RMSD bond length | 0.002 |
RMSD bond angle | 0.503 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.12) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 57.351 | 57.351 | 2.670 |
High resolution limit [Å] | 2.599 | 11.620 | 2.600 |
Rmerge | 0.150 | 0.037 | 1.439 |
Rmeas | 0.178 | 0.045 | 1.707 |
Number of reflections | 34690 | 389 | 2484 |
<I/σ(I)> | 7.53 | 26.32 | 0.91 |
Completeness [%] | 96.8 | 95.1 | 95 |
Redundancy | 3.45 | 3.141 | 3.433 |
CC(1/2) | 0.991 | 0.997 | 0.320 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 0.2 M sodium formate, 20% PEG 3350 |