7PYG
Structure of LPMO in complex with cellotetraose at 3.6x10^5 Gy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-29 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 125.670, 125.670, 125.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.470 - 1.900 |
| R-factor | 0.2456 |
| Rwork | 0.243 |
| R-free | 0.29290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ach |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 50564 | 3763 |
| <I/σ(I)> | 20.25 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.26 | |
| CC(1/2) | 1.000 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 298 | 3.3 M NaCl, 0.1 M Citric acid pH 3.5 |
