7PJP
Crystal structure of YTHDC1 with compound DHU_DC1_226
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-16 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.730, 103.840, 41.950 |
Unit cell angles | 90.00, 106.13, 90.00 |
Refinement procedure
Resolution | 40.300 - 1.610 |
R-factor | 0.1999 |
Rwork | 0.199 |
R-free | 0.22810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r3h |
RMSD bond length | 0.007 |
RMSD bond angle | 0.859 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.300 | 1.700 |
High resolution limit [Å] | 1.610 | 1.610 |
Number of reflections | 41630 | 6576 |
<I/σ(I)> | 11.49 | |
Completeness [%] | 98.0 | |
Redundancy | 3.44 | |
CC(1/2) | 0.998 | 0.683 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5 |