7PB7
Structure of LPMO domain of colonization factor GbpA from Vibrio cholerae in the presence of calcium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-16 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.979655 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.610, 45.200, 99.590 |
Unit cell angles | 90.00, 101.06, 90.00 |
Refinement procedure
Resolution | 32.580 - 1.800 |
R-factor | 0.1813 |
Rwork | 0.179 |
R-free | 0.22820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xwx |
RMSD bond length | 0.005 |
RMSD bond angle | 0.719 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.270 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 33236 | 5267 |
<I/σ(I)> | 7.78 | 1.75 |
Completeness [%] | 99.5 | 98.5 |
Redundancy | 4.1 | 4.1 |
CC(1/2) | 0.993 | 0.705 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Calcium chloride dihydrate, 0.1 M HEPES pH 7.0, 20 % w/v PEG 6000 |