7PB6
Structure of LPMO domain of colonization factor GbpA from Vibrio cholerae in the presence of potassium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-04 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9537 |
Spacegroup name | P 43 |
Unit cell lengths | 46.580, 46.580, 157.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.090 - 1.500 |
R-factor | 0.1655 |
Rwork | 0.164 |
R-free | 0.19230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xwx |
RMSD bond length | 0.007 |
RMSD bond angle | 0.804 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.090 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 52629 | 7798 |
<I/σ(I)> | 28.48 | 8.74 |
Completeness [%] | 98.5 | 90.8 |
Redundancy | 13.4 | 10.9 |
CC(1/2) | 0.999 | 0.973 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Potassium thiocyanate, 30 % w/v PEG 2000 MME |