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7PA6

JC polyomavirus VP1 in complex with scFv 27C11

This is a non-PDB format compatible entry.
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06SA
Synchrotron siteSLS
BeamlineX06SA
Temperature [K]93
Detector technologyPIXEL
Collection date2016-04-08
DetectorDECTRIS PILATUS 2M-F
Wavelength(s)0.99
Spacegroup nameP 1
Unit cell lengths96.090, 96.110, 190.410
Unit cell angles100.91, 93.01, 113.83
Refinement procedure
Resolution47.809 - 1.900
Rwork0.183
R-free0.21640
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3nxg 4kq3
RMSD bond length0.010
RMSD bond angle1.604
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0258)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]48.5002.010
High resolution limit [Å]1.9001.900
Rmeas0.1001.190
Number of reflections465782252959
<I/σ(I)>9.271.15
Completeness [%]89.9
Redundancy3.83
CC(1/2)0.9900.500
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP293.15100 mM potassium phosphate monohydrate / potassium phosphate dihydrate pH 6.0, 10% (w/v) PEG 8000, 686 mM NaCl

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