7P17
F(M197)H mutant structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides strain RV by fixed-target serial synchrotron crystallography (room temperature, 12keV)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 102.500, 102.500, 237.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.050 - 2.220 |
R-factor | 0.1785 |
Rwork | 0.177 |
R-free | 0.21480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6z1j |
RMSD bond length | 0.007 |
RMSD bond angle | 0.971 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.050 | 2.270 |
High resolution limit [Å] | 2.220 | 2.220 |
Rmerge | 0.341 | 2.240 |
Number of reflections | 63262 | 4392 |
<I/σ(I)> | 5 | 0.9 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.8 | 8.3 |
CC(1/2) | 0.972 | 0.339 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 1-mono-oleyl-rac-glycerol (MO), 1,2,3-heptanetriol, Jeffamine M600, N,N-dimethyldodecylamine-N-oxide (LDAO) |