7OWR
HsNMT1 in complex with both MyrCoA and peptide GGKSFSKPR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-06 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98012 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.384, 61.363, 158.871 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 48.370 - 2.390 |
| R-factor | 0.1756 |
| Rwork | 0.173 |
| R-free | 0.21870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o9t |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.823 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.373 | 2.436 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rpim | 0.041 | 0.230 |
| Number of reflections | 34542 | 1728 |
| <I/σ(I)> | 12.69 | 3.122 |
| Completeness [%] | 99.4 | 91.28 |
| Redundancy | 5.71 | 4.92 |
| CC(1/2) | 0.996 | 0.851 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 16% PEG6K, 100mM Sodium Acetate 4.6 |
