7O4E
The DYW domain of A. thaliana OTP86 in its inactive state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.282 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 124.570, 30.580, 77.530 |
Unit cell angles | 90.00, 125.75, 90.00 |
Refinement procedure
Resolution | 38.722 - 2.500 |
R-factor | 0.2241 |
Rwork | 0.222 |
R-free | 0.27140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | D_129211461 |
Data reduction software | XDS (January 26th, 2018) |
Data scaling software | XDS (January 26th, 2018) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmeas | 0.235 | 1.899 |
Number of reflections | 16019 | 1761 |
<I/σ(I)> | 7.46 | 1.06 |
Completeness [%] | 99.2 | 99 |
Redundancy | 6.5 | 6 |
CC(1/2) | 0.994 | 0.428 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 100 nl protein plus 100 nl reservoir (0.1 M glycine, pH 10.5, 1.2 M NaH2PO4, 0.8 M K2HPO4, 0.2 M Li2SO4) plus 30 nl additive (0.1 M 50% v/v Jeffamine M-600 pH 7.0). Cryo: reservoir solution supplemented with 15% (v/v) ethylene glycol. |