EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | DIRECT ELECTRON DE-64 (8k x 8k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.35 |
| Number of Particle | 14778 |
| Software | cisTEM,Auto3DEM |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
