7MXS

Crystal structure of human exonuclease 1 Exo1 (WT) in complex with 5' recessed-end DNA (cr)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-29
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	73.553, 73.553, 181.237
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.577 - 2.798
R-factor	0.2156
Rwork	0.213
R-free	0.24730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5v05
RMSD bond length	0.004
RMSD bond angle	0.902
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.798	2.800
Rmerge	0.067	0.677
Number of reflections	12688	613
<I/σ(I)>	28.93
Completeness [%]	97.4
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		290	100 mM sodium acetate, pH 7.0; 10 mM KCl; 2-4% PEG 4000