7MRJ
Crystal structure of a novel ubiquitin-like TINCR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.676, 67.676, 89.528 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.990 - 2.120 |
R-factor | 0.1928 |
Rwork | 0.188 |
R-free | 0.23620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hwi |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.990 | 2.210 |
High resolution limit [Å] | 2.120 | 2.120 |
Rmerge | 0.140 | 0.660 |
Number of reflections | 12340 | 1186 |
<I/σ(I)> | 15 | |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 24.4 | |
CC(1/2) | 0.980 | 0.830 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5 | 277 | 0.1 M Magnesium sulfate heptahydrate, 0.1 M Sodium Acetate, and 20% (w/v) PEG 1000 |