7MKT

Crystal structure of r(GU)11G-NMM complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-27
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.1271
Spacegroup name	P 1 21 1
Unit cell lengths	42.475, 42.483, 21.607
Unit cell angles	90.00, 90.09, 90.00

Refinement procedure

Resolution	21.610 - 1.970
R-factor	0.27053
Rwork	0.269
R-free	0.29145
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	1.072
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.610	2.040
High resolution limit [Å]	1.970	1.970
Rmerge	0.052	0.755
Rmeas	0.057	0.817
Rpim	0.023	0.308
Number of reflections	5407	529
<I/σ(I)>	16.09	1.57
Completeness [%]	99.0	99.25
Redundancy	6.7	6.9
CC(1/2)	0.998	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	The RNA smaple was prepared by mixing 1.44mM (GU)11G RNA and 1.44 mM NMM in 10 mM Tris, pH 7.03, and 100 mM KCl. An equal volume of RNA sample solution and reservoir solution were mixed and suspended over the reservoir solution consisting of 0.5 M Na/K tartarate, 0.1 M TrisHCl, pH 8.5, 5 mM NaOH. Cryoprotection: reservoir solution supplemented with 30% ethylene glycol.